Multiparent recombinant inbred line ril crosses are a new type of experimental design. Phytophthora root rot prr, caused by phytophthora sojae, has negative effects on soybean yield in china and can be controlled by identifying germplasm resources with resistance genes. Mccouch, s r and doerge, r w 1995 qtl mapping is rice trends genet 11,482487. Aug 20, 2004 recombinant inbred lines rils can serve as powerful tools for genetic mapping. Genetic analysis and qtl mapping of oil content and seed index using two recombinant inbred lines and two backcross populations in upland cotton. To locate map genes a populatton segregating for such genes is required. The dspr panel, created from initially crossing multiple inbred lines to make a synthetic population that then undergoes multiple generations of recombination before formation of recombinant inbred lines, represents an idealogical midway between traditional qtl mapping and genomewide association mapping.
Simulation of population size and genome saturation level for. Identification of qtls for blast, bacterial blight, and. Qtls but for biparent you must have a wide parent in the traits under investigation and need time to develope these mapping. Pyramiding qtl for multiple lateral branching in cucumber using inbred backcross lines. Performance prediction of f1 hybrids between recombinant inbred lines derived from two elite maize inbred lines 2012.
Department of genetics and plant breeding, uas, dharwad, karnataka, india. The segregation data for the map as of 2001 can be downloaded as a text file or downloaded as an excel spreadsheet from nasc. Quantitative trait locus analysis for kernel width using. Both, iril and ril, populations were developed from zea mays inbred lines.
The rqtl2 software expands the scope of the widely used rqtl software. The dspr panel, created from initially crossing multiple inbred lines to make a synthetic population that then undergoes multiple generations of recombination before formation of recombinant inbred lines, represents an idealogical midway between traditional qtl mapping and genomewide association mapping and has several advantages over these. Recently, members of the complex trait consortium proposed the development of a large panel of eightway rils in the mouse, derived from eight genetically diverse parental strains. The bulked f8 seeds we are distributing from the first 100 lines were supplied to us by the nottingham arabidopsis stock centre. It is a computational tool for complex experimental breeding designs. Genetic mapping of agronomic traits using recombinant. Qtl mapping of stalk bending strength in a recombinant.
Inheritance of fresh seed dormancy in recombinant inbred. Valuation of methodologies for mapping oligogenic trait. Genetic analysis and qtl detection on fiber traits using two. Qtl mapping in new arabidopsis thaliana advanced intercross recombinant inbred lines backgroundeven when phenotypic differences are large between natural or domesticated strains, the underlying genetic basis is often complex, and causal genomic regions need to be identified by quantitative trait locus qtl mapping. An ril is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes. Qtl analysis of al tolerance in recombinant inbred lines of. Recombinant inbred linesrils versus near isogenic lines nils. Genetic dissection of maize plant architecture with an ultra. A recombinant inbred line population of soybean glycine max was utilised to identify the quantitative trait loci qtls determining the response to infection by two rootknot. Qtl mapping using magic arabidopsis recombinant inbred lines. Progeny of 300 individual f 2 seedlings were taken by single seed descent to the f 8 generation. Pdf design and construction of recombinant inbred lines. Csr11 is a salttolerant, indica rice variety bred at central soil salinity research institute cssri, karnal, india. Quantitative trail loci mapping of seed protein and oil composition in.
Recombinant inbred lines for mapping rflp and phenotypic. The collection of 5,000 rils in 25 populations is collectively referred to as the maize nested association mapping nam population and was developed by the molecular and functional diversity of the maize genome project. Their growing popularity stems from their potential for increased mapping. As each ril is an inbred strain, and so can be propagated eternally, a panel of rils has a number of advantages for genetic mapping. The rqtl2 software expands the scope of the widely used rqtl software package to include multiparent populations derived from more than two founder strains, such as the collaborative cross and diversity outbred mice, heterogeneous stocks, and magic plant populations. Frontiers identification and finemapping of a major maize. The objectives of this study were to compare the linkage maps and precision of qtl detection in an intermated recombinant inbred line iril population and a selfed recombinant inbred line ril population. Genetic analysis of multiparent recombinant inbred lines. An ril is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes figure 1. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Although a handful of isolated recombinant inbred ri mouse strains were developed during the 1940s and 1950s, the importance of these genetic constructs was not appreciated by any scientist prior to dr. Valuation of methodologies for mapping oligogenic trait loci. The rflp and sslp linkage maps constructed in this study were found to be in the same order as published maps, implying the reliability of the constructed maps. However, there is no mapping software able to generate actual.
We describe a recombinant inbred line ril population of 161 f5 genotypes for the widest euploid cross that can be made to cultivated sorghum sorghum bicolor using conventional techniques, s. A recombinant inbred line population of tomato and its genetic map 443 derived from a cross between two inbred lines via selfbreeding and singleseed descent breeding approach until homozygosity or near homozygosity is reached 11. Constructing linkage maps, reconstructing haplotypes, estimating linkage disequilibrium and quantitative trait loci qtl mapping in multiparent recombinant inbred lines designs. The formulas of haldane and waddington equations 1 have been the basis for linkage analysis in rils for genetic mapping and qtl detection. Registration of the essex x forrest recombinant inbred line mapping population. Mp8, nsl 520824 map recombinant inbred line mapping population was developed through single seed descent from the f 2 generation to the f 5 generation. Mapping quantitative trait loci controlling seed dormancy and heading date in rice, oryza sativa l. Linkage analysis and map construction in plants were first performed in genetically segregating populations derived from two inbred parental lines, such as f 2, backcross, doubled haploid, and recombinant inbred line populations.
Careful attention has been paid to meeting the practical needs of potential users. Intermated recombinant inbred lines irils in plants. Rflp and sslp analyses were conducted to construct linkage maps based on an f8 recombinant inbred line ril mapping population. Qtl quantitative trait loci mapping is commonly used to identify genetic regions responsible to important phenotype variation. The methods and software described here are based around 1200 snp genotypes. Sv map the present sv map derived from dna deep resequencing data of the. In this study, a recombinant inbred line ril population derived from a cross between mo17 a representative inbred line. Recombinant inbred ri lines are particularly useful in genetic mapping studies of quantitative traits. The lgxsm ri strain panel consists of 19 ri lines and provides resource for mapping the genetic basis of complex traits related to obesity and diabetes 38. A common strategy of qtl mapping is to use recombinant inbred lines rils, which are usually established by several generations of inbreeding of an f1 population usually up to f6 or f7 populations. Canadian journal of plant science canadian science publishing. Recombinant inbred lines rils have proven powerful for qtl mapping.
Sorghum propinquum, that segregates for many traits related to plant architecture, growth and development, reproduction, and life history. Recombinant inbred linesrils versus near isogenic lines. Already, they are being implemented in mice complex trait consortium, 2004, arabidopsis kover et al. Seed stocks for tdna insertion lines, natural accesssions, f2 mapping populations, recombinant inbred lines rils, ems mutant homozygous lines,cytolines, heterologous inbred families hifs, and epigenetic recombinant inbred lines. Irils are powerful for highresolution genetic mapping.
Mutant and mapping resources arabidopsis community. Their growing popularity stems from their potential for increased mapping resolution and their ability to capture a wide range of genetic and phenotypic diversity from a breeding population. A recombinant inbred strain or recombinant inbred line is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strains. These two rice lines were selected as donors for biotic resistance in breeding programs for improving rice cultivation in rainfed lowland area in ne. The genomes of recombinant inbred lines complex trait. As each ril is an inbred strain, and so can be propagated eternally, a panel of. Recombinant inbred ri lines of arabidopsis thaliana arabidopsis have been generated from a cross between the ecotypes landsberg erecta and columbia. Recombinant inbred lines for mapping rflp and phenotypic markers in arabidopsis thaliana. A recombinant inbred line population of tomato and its genetic map 445 2. Pdf mapping of qtls for grain yield in wheat recombinant. Here, we constructed a bin map for 670 recombinant inbred lines rils using 1. Among these populations, recombinant inbred lines rils have the special. Mapping qtls for salt tolerance in rice oryza sativa l. The use of recombinant inbred lines rils for genetic mapping article pdf available in methods in molecular biology 82.
Inbred backcross ibc lines and populations plant breeding. Recombinant inbred line rils population development in maize genomics lab. Genetic analysis of rust and late leaf spot in advanced generation recombinant inbred lines of groundnut arachis hypogaea l. Intermated recombinant inbred lines irils in plants, or advanced recombinant inbred strains in animals, are constructed by carrying out generations of intermating between f2 individuals before starting recurrent inbreeding generations by selfing or sibmating. In addition, the identification of quantitative trait loci qtls has been resolutionlimited, with cloning and fine mapping rarely performed. Rqtl is principally aimed at the analysis of simple crosses particularly backcrosses and intercrosses derived from a pair of inbred strains. In the present study, therefore, the objectives were 1 to identify bin markers from highthroughput gbs data in a set of 314 recombinant inbred lines rils derived from two maize elite inbred lines ye478 and qi319 in china. The ilsxiss recombinant inbred strains is a significantly larger ri set consisting of more than 60 lines. Genotyping by sequencing of 393 sorghum bicolor btx623. A population of 268 f 12 derived recombinant inbred lines rils from the. The use of recombinant inbred lines rils for genetic mapping.
Recombinant inbred ri strains are formed by crossing two inbred strains and obtaining a genetically segregating population, perhaps an f 2 or more advanced hybrid cross, then establishing new inbred lines. Both, iril and ril, populations were developed from zea mays inbred lines cg60 and cg102. The objectives of this work were to characterize 154 recombinant inbred lines of common bean by evaluation of quantitative, morphologic, molecular and disease resistance characteristics and to map. Qtl mapping in new arabidopsis thaliana advanced intercross.
Material and methods plant materials hybridizations were made between s. Such a panel would be a valuable community resource. In this video paul andersen explains how the frequency of recombination between linked genes can be used to determine the relative location of genes on a. Imputation and association analysis in arabidopsis thaliana magic recombinant inbred lines beta version. Comparative analysis of metabolome of rice seeds at three. The use of recombinant inbred lines rils for genetic. Parent strains are crossed to create recombinants that. In this video paul andersen explains how the frequency of recombination between linked genes can be used to determine the relative location of genes on a chromosome. In particular, to our knowledge, they are the only core formulas used by genetic mapping and qtl detection software to accommodate data from recombinant inbred lines.
The availability of the recombinant inbreds and the associated data base constitute an efficient means of mapping new molecular markers in maize. Recombinant inbred lines of maize have been developed for the rapid mapping of molecular probes to chromosomal location. They can be derived either by repeated selfing or by repeated sibling brothersister mating from the offspring of an f1 cross between two inbred lines. Dynamic qtl mapping for plant height in upland cotton gossypium hirsutum. Joinmap 4, software for the calculation of genetic linkage maps in. Linkage map construction and quantitative trait loci qtl mapping. In this study, the resistance locus rpshn in the soybean line meng8206 was mapped using two mapping populations.
Jan 01, 20 we describe a recombinant inbred line ril population of 161 f5 genotypes for the widest euploid cross that can be made to cultivated sorghum sorghum bicolor using conventional techniques, s. Recombinant inbred lines rils are very useful in qtl analysis as they represent unique combinations of parental genotypes, and being. Recombinant inbred line rils population development in. Journal of plant registrations abstract mapping population. Initial mapping was realized using two recombinant inbred line. Any genetic study requires one or several genetically segregating populations. Ordas b, malvar r, santiago r, butron a 2010 qtl mapping for mediterranean corn borer resistance in european flint germplasm using recombinant inbred lines.
Recombinant inbred lines how is recombinant inbred lines. Mapping qtl for agronomic traits on wheat chromosome 3a. Genetic analysis of recombinant inbred lines for sorghum. The afrc funded the commercial bulking of these lines. Nested association mapping nam recombinant inbred lines. Pdf characterization of recombinant inbred lines and qtl. Two and threelocus tests for linkage analysis using.
Rqtl includes the ability to analyze doubled haploids or a panel of recombinant inbred lines or even a phaseknown fourway. Linkage analysis with recombinant inbred lines springerlink. This document describes in detail how to install and run software to map qtls in the panel of 703 arabidopsis magic recombinant inbred lines. Recombinant inbred lines rils can serve as powerful tools for genetic mapping. April mapping qtls for salt tolerance in rice oryza sativa l.
F1 and f2 generations are produced by intercrossing the inbred strains. As this inbreeding process involves a large amount of labor, we. To identify biotic stress resistance factors in both rice lines, two recombinant inbred lines rils were developed and used as mapping. This page contains resources relating to the magic population of recombinant inbred line of arabidopsis thaliana, with a focus on data and software to perform genetic association. Intermated recombinant iinbred lines irils in plants liu et al. Qtl mapping for the remaining 10 traits led to traitspecific qtls as well as qtls. Design and construction of recombinant inbred lines article pdf available in methods in molecular biology clifton, n. The phenotypes of six cellwallrelated traits were recorded, and qtl analysis was performed with a genetic map constructed with snp markers. The genetic basis of plant architecture in 10 maize. To identify biotic stress resistance factors in both rice lines, two recombinant inbred lines rils were developed and used as mapping populations. Design and construction of recombinant inbred lines pubmed. Two recombinant inbred families have been constructed from fp populations of t232 x cm37 and c0159 tx303.
In the present study, a highoil inbred line by804 was crossed as male parent with the maize inbred line b73 to construct a population of 188 recombinant inbred lines rils. The magic lines are described in our paper a multiparent advanced generation intercross to fine map. Rils population is used for various mapping studies. Among populations used in plant genetic studies, such as f 2, backcross bc, doubled haploids dh, and recombinant inbred lines ril, two categories can be defined. However, nasc no longer maintains adds new markers this map.
Broman1 department of biostatistics, johns hopkins university, baltimore, maryland 21205 manuscript received august 20, 2004 accepted for publication november 5, 2004 abstract recombinant inbred lines rils can serve as powerful tools for genetic mapping. Imputation analysis of magic arabidopsis thaliana recombinant. Genetic analysis of rust and late leaf spot in advanced. Niu j, guo n, sun j, li l, cao y, li s, huang j, zhao j, zhao t and xing h 2017 fine mapping of a resistance gene rpshn that controls phytophthora sojae using recombinant inbred lines and secondary populations. In many cases, a specrfic population 1s constructed to map a particular trait or locus, e. Donald bailey who first conceptualized their potential utility for linkage analysis while. Physical mapping data are available through gnpgenome software for. Rqtl2 is an interactive software environment for mapping quantitative trait loci qtl in experimental populations. A mapping population of 216 recombinant inbred lines rils was developed from a cross between rice varieties csr11 and mi48 using single seed descent method. To this end, we constructed 10 recombinant inbred line maize populations, designated as an randomopenparent association mapping population xiao et al. Rflp and sslp mapping of salinity tolerance genes in. Quantitative trait loci mapping of meloidogyne incognita. The use of such eightway rils will require a detailed understanding of the. Genetic analysis and qtl mapping of stalk cell wall.
Mapping of qtls for grain yield in wheat recombinant inbred lines. Analysis of natural allelic variation in arabidopsis using a. Sep 01, 2016 recently, a population of 178 recombinant inbred lines rils was developed from a cross between g. The origins and history of recombinant inbred strains are described by crow. Ril populations have been developed and used for numerous studies in many. Rqtl includes the ability to analyze doubled haploids or a panel of recombinant inbred lines. Today, recombinant inbred strains represent a critical tool in the arsenal used to map mouse genes and the ri approach has made its way into the study of other experimental organisms as well such as the. Frontiers fine mapping of a resistance gene rpshn that. Recombinant inbred strain an overview sciencedirect topics. Recombinant inbred lines rils are a collection of strains that can be used to map quantitative trait loci. Nov 14, 2019 mapping quantitative trait loci controlling seed dormancy and heading date in rice, oryza sativa l. Recombinant inbred line mapping population james h. We describe a genetic map with a total of 381 bins of 616 genotyping by sequencing gbsbased snp markers in a f6f8 recombinant inbred line ril population of 393 individuals derived from crossing s. Dec 15, 2002 in the present work, we have analyzed the qtls that control al tolerance in a set of recombinant inbred lines ri lines of arabidopsis made by a cross between landsberg erecta ler and colombia col by lister and dean 1993.
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